CXCL7 antibody

Catalog No. ABIN5689091

This anti-CXCL7 antibody is a Rabbit Polyclonal antibody detecting CXCL7 in WB, ELISA and Neut. Suitable for Human.

Quick Overview for CXCL7 antibody (ABIN5689091)

Target: CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CXCL7 Antibody

Purification: Anti-hNAP-2 specific antibody was purified by affinity chromatography employing immobilized hNAP-2 matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNAP-2 (human Neutrophil Activating Protein-2).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] oof the biological activity of hNAP-2 (50.00 ng/mL), a concentration of 1.75 - 3.0 μ,g/mL of this antibody is required.

ELISA:

To detect hNAP-2 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hNAP-2.

Sandwich:

To detect hNAP-2 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human NAP-2 (XP-5244Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNAP-2.

Western Blot:
To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: NAP-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CXCL7

Target: CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))

Alternative Name: NAP-2

Background: NAP2 is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and sythesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.

Gene ID: 5473

UniProt: P02775