CCL21 antibody

Catalog No. ABIN5689130

This anti-CCL21 antibody is a Rabbit Polyclonal antibody detecting CCL21 in WB, ELISA and Neut. Suitable for Mouse.

Quick Overview for CCL21 antibody (ABIN5689130)

Target: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL21 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL21 Antibody

Purification: Anti-mExodus-2 specific antibody was purified by affinity chromatography employing immobilized mExodus-2 matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mExodus-2 (murine Exodus-2).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mExodus-2 (100.0 ng/mL), a concentration of 9.0-10.0 μ,g/mL of this antibody is required.

ELISA:

To detect mExodus-2 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mExodus-2.

Sandwich: To detect mExodus-2 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine Exodus-2 (XP-5129Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mExodus-2.

Western Blot:

To detect mExodus-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mExodus-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: Exodus-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CCL21

Target: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Alternative Name: Exodus-2

Background: Exodus 2 is one of several CC cytokine genes clustered on the p arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterised by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils.

Gene ID: 65956

UniProt: P86793

Pathways: Regulation of Actin Filament Polymerization