IL-7 antibody

Catalog No. ABIN5689150

The Rabbit Polyclonal anti-IL-7 antibody has been validated for WB, ELISA and Neut. It is suitable to detect IL-7 in samples from Mouse.

Quick Overview for IL-7 antibody (ABIN5689150)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL-7 Antibody

Purification: Anti-mIL-7 specific antibody was purified by affinity chromatography employing immobilized mIL-7 matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-7 (murine IL-7).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-7 (0.60 ng/mL), a concentration of 0.012 - 0.02 μ,g/mL of this antibody is required.

ELISA:

To detect mIL-7 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-7.

Sandwich:

To detect mIL-7 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IL-7 (XP-5198Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-7.

Western Blot:

To detect mIL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: IL-7 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Alternative Name: IL-7

Background: Iterleukin 7 is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL-7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL-7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL-7 stimulates the proliferation of CD4+/CD8+ cells.

Gene ID: 16196

UniProt: P10168

Pathways: JAK-STAT Signaling