Ccl12 antibody

Catalog No. ABIN5689163

This anti-Ccl12 antibody is a Rabbit Polyclonal antibody detecting Ccl12 in ELISA, WB and Neut. Suitable for Mouse.

Quick Overview for Ccl12 antibody (ABIN5689163)

Target: Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ccl12 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Neutralization (Neut)

Product Details anti-Ccl12 Antibody

Purification: Anti-mMCP-5 specific antibody was purified by affinity chromatography employing immobilized mMCP-5 matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMCP-5 (murine MCP-5).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mMCP-5 (100 ng/mL), a concentration of 2.0 - 3.0 μ,g/mL of this antibody is required.

ELISA:

To detect mMCP-5 by direct ELISA (using 100 μ,g/mL of this μ,L/well antibody solution) a concentration of at least 0.5 antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMCP-5.

Sandwich:

To detect mMCP-5 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine MCP-5 (XP-5226Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-5.

Western Blot:

To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: MCP-5 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for Ccl12

Target: Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))

Alternative Name: MCP-5

Background: MCP-5 is a member of the b-chemokine family (C-C) of cytokines. This chemokine is strongly chemotactic for human monocytes and murine peritoneal macrophages, but is not active on mouse neutrophils and shows only a weak response on mouse eosinophils at high concentrations

Gene ID: 20293

UniProt: Q62401

Pathways: Cellular Response to Molecule of Bacterial Origin