CCL9 antibody (Biotin)

Catalog No. ABIN5689166

This anti-CCL9 antibody is a Rabbit Polyclonal antibody detecting CCL9 in WB and ELISA. Suitable for Mouse.

Quick Overview for CCL9 antibody (Biotin) (ABIN5689166)

Target: CCL9 (Ccl9) (Chemokine (C-C Motif) Ligand 9 (Ccl9))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL9 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-CCL9 Antibody (Biotin)

Purification: Anti-mMIP-1-gamma specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMIP-1-gamma (murine MIP-1-gamma).

Application Details

Application Notes: ELISA:

To detect mMIP-1-γ, by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mMIP-1-γ,.

Sandwich:

To detect mMIP-1γ, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine MIP-1γ, (XP-5236) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-1γ,.

Western Blot:

To detect mMIP-1-γ, by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-1-γ, is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: MIP-1gamma antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CCL9

Target: CCL9 (Ccl9) (Chemokine (C-C Motif) Ligand 9 (Ccl9))

Alternative Name: MIP-1gamma

Gene ID: 20308

UniProt: P51670