Ephrin B2 antibody (pTyr316)

Catalog No. ABIN5689216

The Rabbit Polyclonal anti-Ephrin B2 antibody has been validated for WB. It is suitable to detect Ephrin B2 in samples from Human and Mouse.

Quick Overview for Ephrin B2 antibody (pTyr316) (ABIN5689216)

Target: Ephrin B2 (EFNB2)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ephrin B2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Ephrin B2 Antibody

Binding Specificity: pTyr316

Specificity: This antibody detects endogenous levels of Ephrin-B2 only when phosphorylated at tyrosine 316.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: Ephrin-B2 (Phospho-Tyr316) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 316 (P-V-Y (p) -I-V) derived from Human Ephrin-B2.

Application Details

Application Notes: Western Blot: 1:500~1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for Ephrin B2

Target: Ephrin B2 (EFNB2)

Alternative Name: Ephrin B2

Background: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.

Molecular Weight: 37 kDa

Gene ID: 1948

NCBI Accession: NP_004084

UniProt: P52799

Pathways: RTK Signaling, Regulation of Muscle Cell Differentiation