HDAC5 antibody (AA 496-500)

Catalog No. ABIN5689288

The Rabbit Polyclonal anti-HDAC5 antibody has been validated for WB and IHC. It is suitable to detect HDAC5 in samples from Human.

Quick Overview for HDAC5 antibody (AA 496-500) (ABIN5689288)

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HDAC5 Antibody

Binding Specificity: AA 496-500

Specificity: This antibody detects endogenous level of total HDAC5 protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: HDAC5 (Ab-498) antibody was raised against a peptide sequence around aa.496~500 (T-Q-S-S-P) derived from Human HDAC5/7.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for HDAC5

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Alternative Name: HDAC5

Background: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC5 belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 124 kDa

Gene ID: 10014

NCBI Accession: NP_001015053

UniProt: Q9UQL6

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Monocarboxylic Acid Catabolic Process