Nanog antibody (AA 137-141)

Catalog No. ABIN5689402

The Rabbit Polyclonal anti-Nanog antibody has been validated for WB. It is suitable to detect Nanog in samples from Human and Mouse.

Quick Overview for Nanog antibody (AA 137-141) (ABIN5689402)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Nanog Antibody

Binding Specificity: AA 137-141

Specificity: This antibody detects endogenous level of total Nanog protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: Nanog antibody was raised against a peptide sequence around aa.137~141 (K-Q-V-K-T) derived from Nano g.

Application Details

Application Notes: Western Blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: Nanog

Background: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes By similarity. Acts as a transcriptional activator or repressor By similarity. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' By similarity. When overexpressed, promotes cells to enter into S phase and proliferation

Molecular Weight: 42 kDa

Gene ID: 79923

NCBI Accession: NP_079141

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance