EIF4E antibody (AA 207-211)

Catalog No. ABIN5689452

This Rabbit Polyclonal antibody specifically detects EIF4E in WB, IHC and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for EIF4E antibody (AA 207-211) (ABIN5689452)

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4E antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-EIF4E Antibody

Binding Specificity: AA 207-211

Specificity: This antibody detects endogenous level of total elF4E protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: eIF4E (Ab-209) antibody was raised against a peptide sequence around aa. 207~211 (S-G-S-T-T) derived from Human eIF4E.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for EIF4E

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Alternative Name: eIF4E

Background: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Molecular Weight: 25 kDa

Gene ID: 1977

NCBI Accession: NP_001124150

UniProt: P06730

Pathways: BCR Signaling