VASP antibody (AA 155-159)

Catalog No. ABIN5689501

The Rabbit Polyclonal anti-VASP antibody is suitable to detect VASP in samples from Human and Rat. It has been validated for WB and IF.

Quick Overview for VASP antibody (AA 155-159) (ABIN5689501)

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VASP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-VASP Antibody

Binding Specificity: AA 155-159

Specificity: This antibody detects endogenous level of total VASP protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: VASP (Ab-157) antibody was raised against a peptide sequence around aa.155~159 (R-V-S-N-A) derived from Human VASP.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for VASP

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Alternative Name: VASP

Background: Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.

Molecular Weight: 50 kDa

Gene ID: 7408

NCBI Accession: NP_003361

UniProt: P50552

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation