Caspase 3 antibody (AA 29-33)

Catalog No. ABIN5689549

The Rabbit Polyclonal anti-Caspase 3 antibody has been validated for WB. It is suitable to detect Caspase 3 in samples from Human.

Quick Overview for Caspase 3 antibody (AA 29-33) (ABIN5689549)

Target: Caspase 3 (CASP3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Caspase 3 Antibody

Binding Specificity: AA 29-33

Specificity: This antibody detects endogenous level of total Caspase 3 protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: Caspase 3 antibody was raised against a peptide sequence around aa.29~33 (S-G-I-S-L) derived from Caspase 3.

Application Details

Application Notes: Western Blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: Caspase 3

Background: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.

Molecular Weight: 35 19 17 kDa

Gene ID: 836

NCBI Accession: NP_004337

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation