LIN28A antibody (AA 1-5)

Catalog No. ABIN5689572

This Rabbit Polyclonal antibody specifically detects LIN28A in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for LIN28A antibody (AA 1-5) (ABIN5689572)

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIN28A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LIN28A Antibody

Binding Specificity: AA 1-5

Specificity: This antibody detects endogenous levels of total Lin28 protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: Lin28 antibody was raised against a peptide sequence around aa. 1~5 (M-G-S-V-S) derived from Human Lin28 .

Application Details

Application Notes: Western Blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for LIN28A

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Alternative Name: Lin28

Background: Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells.

Molecular Weight: 26 kDa

Gene ID: 79727

NCBI Accession: NP_078950

UniProt: Q9H9Z2

Pathways: Stem Cell Maintenance