ABL1/2 antibody (pTyr393, pTyr429)

Catalog No. ABIN5689742

The Rabbit Polyclonal anti-ABL1/2 antibody (ABIN5689742) specifically detects ABL1/2 in WB, IHC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for ABL1/2 antibody (pTyr393, pTyr429) (ABIN5689742)

Target: ABL1/2 (ABL1/ABL2) (Abelson Murine Leukemia Viral Oncogene Homolog 1/2 (ABL1/ABL2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABL1/2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ABL1/2 Antibody

Binding Specificity: pTyr393, pTyr429

Specificity: This antibody detects endogenous level of ABL1/2 only when phosphorylated at tyrosine393/429.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Immunogen: ABL1/2 (phospho-Tyr393/429) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 393/429 (D-T-Y (p) -T-A) derived from Human ABL1/2.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for ABL1/2

Target: ABL1/2 (ABL1/ABL2) (Abelson Murine Leukemia Viral Oncogene Homolog 1/2 (ABL1/ABL2))

Alternative Name: ABL1 / 2

Background: Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.

Molecular Weight: 210 kDa

Gene ID: 25, 27

NCBI Accession: NP_005148, NP_001129472

UniProt: P00519, P42684