Ezrin antibody (pTyr353)

Catalog No. ABIN5689844

This anti-Ezrin antibody is a Rabbit Polyclonal antibody detecting Ezrin in WB and IHC. Suitable for Human and Mouse.

Quick Overview for Ezrin antibody (pTyr353) (ABIN5689844)

Target: Ezrin (EZR)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ezrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Ezrin Antibody

Binding Specificity: pTyr353

Specificity: This antibody detects endogenous level of Ezrin only when phosphorylated at tyrosine 353.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Immunogen: Ezrin (Phospho-Tyr353) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 353 (Q-D-Y (p) -E-E) derived from Human EZRIN.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for Ezrin

Target: Ezrin (EZR)

Alternative Name: Ezrin

Background: Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

Molecular Weight: 81 kDa

Gene ID: 7430

NCBI Accession: NP_001104547

UniProt: P15311

Pathways: Maintenance of Protein Location