APOE antibody (AA 220-270)

Catalog No. ABIN5689876

The Rabbit Polyclonal anti-APOE antibody has been validated for WB, ELISA, IHC (p) and IF. It is suitable to detect APOE in samples from Human, Mouse and Rat.

Quick Overview for APOE antibody (AA 220-270) (ABIN5689876)

Target: APOE (Apolipoprotein E (APOE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-APOE Antibody

Binding Specificity: AA 220-270

Purification: APO-E Antibody is affinity chromatography purified via peptide column.

Immunogen: APO-E antibody was raised against a 19 amino acid peptide near the carboxy terminus of human APO-E.

The immunogen is located within amino acids 220 - 270 of APO-E.

Isotype: IgG

Application Details

Application Notes: APO-E antibody can be used for detection of APO-E by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: APO-E Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: APO-E antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for APOE

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: APO-E

Background: APO-E Antibody: Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E (APO-E), a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. Defects in APO-E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.

Molecular Weight: Predicted: 35 kDa

Observed: 34 kDa

Gene ID: 348

NCBI Accession: NP_000032

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism