Artemin antibody was purified by affinity chromatography and then biotinylated.
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hArtemin. Human Artemin specific antibody was purified by affinity chromatography and then biotinylated.
ARTN
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
ELISA:
Direct: To detect hArtemin by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hArtemin. Sandwich: To detect hArtemin by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required.
Western Blot: To detect hArtemin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hArtemin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Restrictions
For Research Use only
Format
Lyophilized
Storage
-20 °C
Storage Comment
Artemin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Target
ARTN
(Artemin (ARTN))
Alternative Name
Artemin
Background
Artemin has been shown in culture to support the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons. Its role in the PNS and CNS is further substantiated by its expression pattern in the proximity of these neurons.