beta Defensin 1 antibody (Biotin)

Catalog No. ABIN5689900

The Rabbit Polyclonal anti-beta Defensin 1 antibody has been validated for WB and ELISA. It is suitable to detect beta Defensin 1 in samples from Human.

Quick Overview for beta Defensin 1 antibody (Biotin) (ABIN5689900)

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Defensin 1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-beta Defensin 1 Antibody (Biotin)

Purification: BD-1 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBD-1. Human BD-1 specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect hBD-1 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-1.
Sandwich:
To detect hBD-1 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required..

Western Blot:
To detect hBD-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,L/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: BD-1 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for beta Defensin 1

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Alternative Name: BD-1

Gene ID: 1672

UniProt: P60022