IL-22 antibody

Catalog No. ABIN5690008

The Rabbit Polyclonal anti-IL-22 antibody has been validated for WB, ELISA and Neut. It is suitable to detect IL-22 in samples from Human.

Quick Overview for IL-22 antibody (ABIN5690008)

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-22 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL-22 Antibody

Purification: IL-22 specific antibody was purified by affinity chromatography employing immobilized hIL-22 matrix.

Immunogen: E.coli derived Recombinant Human IL-22

Application Details

Application Notes: ELISA:
Sandwich:
To detect hIL-22 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Polyclonal Biotinylated Anti-Human IL-22 (38-142) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-22.

Western Blot:

To detect hIL-22 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-22 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Neutralization:

To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-22 (50.0 ng/mL), a concentration of 1.25 μ,g/mL of this antibody is required.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL-22

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Alternative Name: IL-22

Gene ID: 50616

UniProt: Q9GZX6