BMP7 antibody (Biotin)

Catalog No. ABIN5690376

This Rabbit Polyclonal antibody specifically detects BMP7 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for BMP7 antibody (Biotin) (ABIN5690376)

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BMP7 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-BMP7 Antibody (Biotin)

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBMP-7. Human BMP-7 specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect hBMP-7 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBMP-7.

Sandwich
To detect hBMP-7 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human BMP-7 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBMP-7.

Western Blot:
To detect hBMP-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: BMP-7 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for BMP7

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Alternative Name: BMP-7

Background: BMPs (bone morphogenetic proteins) belong to the TGF beta superfamily of structurally related signaling proteins. Members of this superfamily are widely represented throughout the animal kingdom and have been implicated in a variety of developmental processes. Proteins of the TGF beta superfamily are disulfide-linked dimers composed of two 12-15 kDa polypeptide chains. As implied by their name, BMPs initiate, promote and regulate bone development, growth, remodeling and repair.

Gene ID: 655

UniProt: P18075

Pathways: Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance