Betacellulin antibody (Biotin)

Catalog No. ABIN5690379

This Rabbit Polyclonal antibody specifically detects Betacellulin in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Betacellulin antibody (Biotin) (ABIN5690379)

Target: Betacellulin (BTC)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Betacellulin antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-Betacellulin Antibody (Biotin)

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBetacellulin. Human Betacellulin specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect hBetacellulin by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBetacellulin.

Sandwich
To detect hBetacellulin by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human Betacellulin as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBetacellulin.

Western Blot:
To detect hBetacellulin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBetacellulin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: Betacellulin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for Betacellulin

Target: Betacellulin (BTC)

Alternative Name: Betacellulin

Background: Betacellulin (BTC) is a heparin-binding member of the EGF family of growth factors that includes epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha), amphiregulin (AR), heparin-binding EGF-like growth factor (HB-EGF), and the various heregulins (HRG).

Gene ID: 685

UniProt: P35070

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway