CCL8 antibody (Biotin)

Catalog No. ABIN5690385

This Rabbit Polyclonal antibody specifically detects CCL8 in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for CCL8 antibody (Biotin) (ABIN5690385)

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL8 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-CCL8 Antibody (Biotin)

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMCP-2. Murine MCP-2 specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect mMCP-2 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-2.

Sandwich
To detect mMCP-2 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Murine MCP-2 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-2.

Western Blot:
To detect mMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: MCP-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CCL8

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Alternative Name: MCP-2

Background: MCP 2 is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells.

Gene ID: 20307

UniProt: Q9Z121