IL17F antibody

Catalog No. ABIN5690438

The Rabbit Polyclonal anti-IL17F antibody (ABIN5690438) specifically detects IL17F in WB, ELISA and Neut. The antibody is reactive with Human samples.

Quick Overview for IL17F antibody (ABIN5690438)

Target: IL17F (Interleukin 17F (IL17F))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL17F antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL17F Antibody

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-17F. Human IL-17F specific antibody was purified by affinity chromatography employing immobilized hIL-17F matrix.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-17F (50.0 ng/mL), a concentration of 0.9-1.2 μ,g/mL of this antibody is required.

ELISA:
Indirect:
To detect hIL-17F by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17F.

Sandwich
To detect hIL-17F by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human IL-17F as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17F.

Western Blot:
To detect hIL-17F by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17F is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL17F

Target: IL17F (Interleukin 17F (IL17F))

Alternative Name: IL-17F

Gene ID: 112744

UniProt: Q96PD4

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity