Persephin antibody (Biotin)

Catalog No. ABIN5690449

The Rabbit Polyclonal anti-Persephin antibody has been validated for WB and ELISA. It is suitable to detect Persephin in samples from Human.

Quick Overview for Persephin antibody (Biotin) (ABIN5690449)

Target: Persephin (PSPN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Persephin antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-Persephin Antibody (Biotin)

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPersephin. Human Persephin specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect hPersephin by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPersephin.

Sandwich
To detect hPersephin by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human Persephin as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPersephin.

Western Blot:
To detect hPersephin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPersephin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: Persephin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for Persephin

Target: Persephin (PSPN)

Alternative Name: Persephin

Background: Persephin is a neurotrophic factor, belonging to the GDNF family. Neurotrophic factors are important for the proper development and maintenance of the nervous system. These factors promote neuronal survival and can prevent the neuronal degeneration associated with injury, toxin exposure, or neurodegenerative disease. Persephin has a similar structure to other family members, glial cell line-derived neurotrophic factor, Artemin and neurturin. All these proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures.

Gene ID: 5623

UniProt: O60542