IL17B antibody (Biotin)

Catalog No. ABIN5690469

This Rabbit Polyclonal antibody specifically detects IL17B in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for IL17B antibody (Biotin) (ABIN5690469)

Target: IL17B (Interleukin 17B (IL17B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL17B antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-IL17B Antibody (Biotin)

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-17B. Human IL-17B specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect hIL-17B by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17B.

Sandwich
To detect hIL-17B by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IL-17B as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17B.

Western Blot:
To detect hIL-17B by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17B is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: IL-17B antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL17B

Target: IL17B (Interleukin 17B (IL17B))

Alternative Name: IL-17B

Gene ID: 27190

UniProt: Q9UHF5