IL-19 antibody (Biotin)

Catalog No. ABIN5690470

The Rabbit Polyclonal anti-IL-19 antibody has been validated for WB and ELISA. It is suitable to detect IL-19 in samples from Human.

Quick Overview for IL-19 antibody (Biotin) (ABIN5690470)

Target: IL-19 (IL19) (Interleukin 19 (IL19))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-19 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-IL-19 Antibody (Biotin)

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-19. Human IL-19 specific antibody was purified by affinity chromatography and then biotinylated.ced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-19. Human IL-19 specific antibody was purified by affinity chromatography employing immobilized hIL-19 matrix.

Application Details

Application Notes: ELISA:
Direct:
To detect hIL-19 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-19.

Sandwich
To detect hIL-19 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IL-19 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-19.

Western Blot:
To detect hIL-19 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-19 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: IL-19 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL-19

Target: IL-19 (IL19) (Interleukin 19 (IL19))

Alternative Name: IL-19

Background: IL19 is a cytokine that belongs to the IL10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Little is known about the biologic function and gene regulation of IL19.

Gene ID: 29949

UniProt: Q9UHD0