CCL22 antibody

Catalog No. ABIN5690476

The Rabbit Polyclonal anti-CCL22 antibody is suitable to detect CCL22 in samples from Mouse. It has been validated for WB, ELISA and Neut.

Quick Overview for CCL22 antibody (ABIN5690476)

Target: CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL22 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL22 Antibody

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMDC. Murine MDC specific antibody was purified by affinity chromatography employing immobilized mMDC matrix.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mMDC (100 ng/mL), a concentration of 6-9 μ,g/mL of this antibody is required. ELISA:
Indirect:
To detect mMDC by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMDC.

Sandwich
To detect mMDC by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine MDC as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMDC.

Western Blot:
To detect mMDC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMDC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: MDC antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CCL22

Target: CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

Alternative Name: MDC

Background: The MDC gene is one of several Cys-Cys (CC) cytokine genes which are clustered on the q arm of chromosome 16.

Gene ID: 20299

UniProt: O88430