VEGF antibody (Biotin)

Catalog No. ABIN5690497

The Rabbit Polyclonal anti-VEGF antibody is suitable to detect VEGF in samples from Rat. It has been validated for WB and ELISA.

Quick Overview for VEGF antibody (Biotin) (ABIN5690497)

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEGF antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-VEGF Antibody (Biotin)

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat VEGF (Rat Vascular Endothelial Growth Factor). Rat VEGF specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect Rat VEGF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat VEGF.

Sandwich
To detect Rat VEGF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Rat VEGF as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat VEGF.

Western Blot:
To detect Rat VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat VEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: VEGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for VEGF

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Alternative Name: VEGF

Gene ID: 83785

UniProt: P16612