CCL4 antibody

Catalog No. ABIN5690510

This anti-CCL4 antibody is a Rabbit Polyclonal antibody detecting CCL4 in WB, ELISA and Neut. Suitable for Mouse.

Quick Overview for CCL4 antibody (ABIN5690510)

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL4 Antibody

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMIP-1β,. Murine MIP-1β, specific antibody was purified by affinity chromatography employing immobilized mMIP-1β, matrix.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Murine MIP-1β, (100.0 ng/mL), a concentration of 8.0-10.0 μ,g/mL of this antibody is required.

ELISA:
Indirect:
To detect mMIP-1b by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-1b.

Sandwich
To detect mMIP-1b by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine MIP-1b as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-1b.

Western Blot:
To detect mMIP-1b by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-1b is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: MIP-1B antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CCL4

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Alternative Name: MIP-1B

Gene ID: 20303

UniProt: P14097