Follistatin antibody

Catalog No. ABIN5690528

This anti-Follistatin antibody is a Rabbit Polyclonal antibody detecting Follistatin in WB, ELISA and Neut. Suitable for Human.

Quick Overview for Follistatin antibody (ABIN5690528)

Target: Follistatin (FST)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Follistatin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-Follistatin Antibody

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFollistatin. Human Follistatin specific antibody was purified by affinity chromatography employing immobilized hFollistatin matrix.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human Follistatin (100.0 ng/mL), a concentration of 8.0-12.0 μ,g/mL of this antibody is required.

ELISA:
Indirect:
To detect hFollistatin by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin.

Sandwich
To detect hFollistatin by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Follistatin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin.

Western Blot:
To detect hFollistatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFollistatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: Follistatin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for Follistatin

Target: Follistatin (FST)

Alternative Name: Follistatin

Background: Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. There is evidence linking polycystic ovary syndrome and follistatin. Follistatin binds directly to activin and functions as an activin antagonist. It specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone

Gene ID: 10468

UniProt: P19883

Pathways: Negative Regulation of Hormone Secretion