Follistatin antibody (Biotin)

Catalog No. ABIN5690529

This Rabbit Polyclonal antibody specifically detects Follistatin in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Follistatin antibody (Biotin) (ABIN5690529)

Target: Follistatin (FST)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Follistatin antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-Follistatin Antibody (Biotin)

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFollistatin. Human Follistatin specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect hFollistatin by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin.

Sandwich
To detect hFollistatin by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human Follistatin as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin.

Western Blot:
To detect hFollistatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFollistatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: Follistatin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for Follistatin

Target: Follistatin (FST)

Alternative Name: Follistatin

Background: Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. There is evidence linking polycystic ovary syndrome and follistatin. Follistatin binds directly to activin and functions as an activin antagonist. It specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone

Gene ID: 10468

UniProt: P19883

Pathways: Negative Regulation of Hormone Secretion