MIA2 antibody (Biotin)

Catalog No. ABIN5690596

The Rabbit Polyclonal anti-MIA2 antibody is suitable to detect MIA2 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for MIA2 antibody (Biotin) (ABIN5690596)

Target: MIA2 (Melanoma Inhibitory Activity Protein 2 (MIA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIA2 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-MIA2 Antibody (Biotin)

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIA-2 (human MIA-2). Human MIA-2 specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect hMIA-2 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIA-2.

Sandwich
To detect hMIA-2 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human MIA-2 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIA-2.

Western Blot:
To detect hMIA-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIA-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: MIA-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for MIA2

Target: MIA2 (Melanoma Inhibitory Activity Protein 2 (MIA2))

Alternative Name: MIA-2

Gene ID: 117153

UniProt: Q96PC5