Defensin beta 3 antibody (Biotin)

Catalog No. ABIN5690603

This anti-Defensin beta 3 antibody is a Rabbit Polyclonal antibody detecting Defensin beta 3 in WB and ELISA. Suitable for Human.

Quick Overview for Defensin beta 3 antibody (Biotin) (ABIN5690603)

Target: Defensin beta 3 (DEFB3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Defensin beta 3 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-Defensin beta 3 Antibody (Biotin)

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBD-3. Human BD-3 specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect hBD-3 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-3.

Sandwich
To detect hBD-3 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human BD-3 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-3.

Western Blot:
To detect hBD-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: BD-3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for Defensin beta 3

Target: Defensin beta 3 (DEFB3)

Alternative Name: BD-3

Gene ID: 55894

UniProt: P81534

Pathways: Production of Molecular Mediator of Immune Response