Dynamin 1 antibody (pSer778)

Catalog No. ABIN5690706

This Sheep Polyclonal antibody specifically detects Dynamin 1 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Dynamin 1 antibody (pSer778) (ABIN5690706)

Target: Dynamin 1 (DNM1)

Reactivity: Human, Mouse, Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This Dynamin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Dynamin 1 Antibody

Binding Specificity: pSer778

Purification: Affinity Purified

Immunogen: Dynamin (Ser778) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser778 of human, mouse and rat dynamin.

Application Details

Application Notes: Dynamin antibody for phospho-Ser778 dynamin labels the purified protein phosphorylated in vitro by Cdk5 but not PKC (Tan, et al. 2003). The labeling of the dynamin band in Western blots of rat brain was blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Sheep anti- Dynamin (Ser778) does not cross react with other purified substrates of CDK5 (e.g. amphiphysin and synapsin). Applications include Western Blots (WB). Human, mouse and rat have 100 % amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for WB.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20°C.

Target Details for Dynamin 1

Target: Dynamin 1 (DNM1)

Alternative Name: Dynamin

Background: Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo. Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.

Molecular Weight: 95 kDa

Gene ID: 140694

UniProt: P21575

Pathways: Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling