CD8 antibody

Catalog No. ABIN5691089

This anti-CD8 antibody is a Mouse Monoclonal antibody detecting CD8 in FACS, IHC (fro), ELISA and ICC. Suitable for Human.

Quick Overview for CD8 antibody (ABIN5691089)

Target: CD8

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunocytochemistry (ICC)

Clone: RIV11

Product Details anti-CD8 Antibody

Specificity: The antibody RIV11 is directed against human CD8 and recognises all CD8 positive T-cells, thymocytes and natural killer cells.

Immunogen: RIV11 is a mouse monoclonal IgG1 antibody derived by fusion of 653 mouse myeloma cells with splenocytes from a BALB/c mouse immunized with human peripheral blood lymphocytes.

Isotype: IgG1

Application Details

Application Notes: The RIV11 antibody is suitable for the detection of total CD8 by flow cytometry and ELISA. The antibody is also suitable for the detection of CD8 by immunocytochemistry and immunohistochemistry on frozen tissues. Human lymph node, spleen and peripheral blood lymphocytes are suitable for use as positive controls. Optimal antibody dilutions for the different applications should be determined by titration.

Restrictions: For Research Use only

Handling

Buffer: Each vial contains 500 μL 0.2 mg/mL purified monoclonal antibody in phosphated buffered saline (PBS) containing 50 % glycerol + 0.2 % bovine serum albumin (BSA) + 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C, or in small aliquots at -20°C.

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background: CD8 is a 32 kDa cell surface transmembrane glycoprotein expressed by most thymocytes, a subpopulation of mature peripheral blood T-cells and natural killer (NK) cells. CD8 is expressed on the cell surface as a heterodimer, comprising two distinct disulphide linked α and β polypeptide chains, or as a CD8α homodimer. The CD8 αβ heterodimer is expressed on T-cell receptor (TCR) αβ+ cytotoxic T-lymphocytes (CTL's), whereas CD8α homodimers are expressed on TCR γδ+ T-cells, natural killer cells and a subpopulation of TCR αβ+ T-cells. The CD8 Molecule functions as a co-receptor (with the T-cell receptor) as part of the antigen-recognition complex on TCRαβ+, class I MHC-restricted T cells and plays an important role in T-cell signaling. The CD8 Ig-like domain binds to the alpha-3 domain of MHC-Class 1.

UniProt: P01732