TNC antibody

Catalog No. ABIN5691113

This anti-TNC antibody is a Mouse Monoclonal antibody detecting TNC in WB, IP, IHC (p), ICC and IHC (fro). Suitable for Human.

Quick Overview for TNC antibody (ABIN5691113)

Target: TNC (Tenascin C (TNC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNC antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: T2H5

Product Details anti-TNC Antibody

Specificity: In immunoblotting assays on an extract of a human osteosarcoma cell line (U2OS) T2H5 recognizes a 265 kDa protein. Tenascin C contains an exstensive number of potential glycosylation sites, which may explain its migration at a higher molecular weight than predicted. Human tonsilar tissue can be used as a positive control in immunocytochemistry and immunohistochemistry.

Immunogen: T2H5 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a BALB/c mouse immunized with full length native tenascin protein purified from a homogenate of a human mammary tumour specimen.

Isotype: IgG1

Application Details

Application Notes: T2H5 is suitable for immunoblotting, immunocytochemistry, and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Buffer: Each vial contains 100 μL 1 mg/mL purified monoclonal antibody in PBS containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C, or in small aliquots at -20°C.

Target Details for TNC

Target: TNC (Tenascin C (TNC))

Alternative Name: Tenascin C

Background: Tenascin is a high molecular weight, multifunctional, extracellular matrix glycoprotein expressed in association with mesenchymal-epithelial interactions during development. It has been described under a variety of names, i.e. cytotactin, hexabrachion protein, J1, myotendinous antigen and glioma mesenchymal extracellular matrix. In undifferentiated tumors it is also detectable in the neovasculature and stroma. The tenascin molecule is a disulfide-linked hexamer. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted. For example, this extracellular matrix protein is implicated in guidance of migrating neurons as well as axons during neural development as well as neuronal regeneration. In in vitro studies it promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. The cellular ligands for tenascin include integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development