CD39 antibody

Catalog No. ABIN5691127

The Rat Monoclonal anti-CD39 antibody has been validated for FACS. It is suitable to detect CD39 in samples from Human.

Quick Overview for CD39 antibody (ABIN5691127)

Target: CD39 (ENTPD1) (Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This CD39 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: R22

Product Details anti-CD39 Antibody

Specificity: The antibody R22 is directed against human CD39. The antibody has not been tested for cross reactivity with other species.

Immunogen: R22 is a rat monoclonal IgG1 antibody derived by fusion of SP2/0 mouse myeloma cells with splenocytes from a WAG/Rij rat immunized with JY cells, a human B lymphoblastoid cell line.

Isotype: IgG1

Application Details

Application Notes: The R22 antibody is suitable for the detection of human CD39 antigen by flow cytometry. Human spleen is suitable as a positive control. Optimal antibody dilutions for the different applications should be determined by titration.

Restrictions: For Research Use only

Handling

Buffer: Each vial contains 100 μL 1 mg/mL purified monoclonal antibody in phosphate buffered saline (PBS) containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C, or in small aliquots at -20°C.

Target Details for CD39

Target: CD39 (ENTPD1) (Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1))

Alternative Name: CD39, Ectonucleoside triphosphate diphosphohydrolase 1

Background: CD39 is a transmembrane cell surface protein, which is a member of the ecto-nucleoside triphosphate diphosphohydrolase (ENTPDase) family. The CD39 Molecule is primarily involved in the metabolism of purine nucleotides, converting extracellular adenosine triphosphate (ATP) and adenosine diphosphate (ADP) to adenosine monophosphate (AMP). In conjunction with CD73 (5' nucleotidase), CD39 plays a key role in regulating immunity and inflammation through the production of adenosine and purinergic signalling pathways. In addition, CD39 is reported to mediate B cell homotypic adhesion and inhibit ADP-induced platelet aggregation. CD39 was originally identified on Epstein Barr virus transformed B cell lines and described as a B-cell activation marker. However, subsequent studies have shown that CD39 is also expressed on activated T-cells, T-regulatory cells, natural killer cells, tissue macrophages and endothelial cells. Increased expression of CD39 has also been reported in cases of chronic lymphocytic leukaemia and several solid tumours including colorectal and pancreatic cancer suggesting a potential role for CD39 in tumour development and progression.

UniProt: P49961