Histone H3.3 antibody (G34V)

Catalog No. ABIN5692159

The Mouse Monoclonal anti-Histone H3.3 antibody has been validated for IHC (p). It is suitable to detect Histone H3.3 in samples from Human.

Quick Overview for Histone H3.3 antibody (G34V) (ABIN5692159)

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H3.3 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Clone: 329E5

Product Details anti-Histone H3.3 Antibody

Binding Specificity: AA 29-38, G34V

Purpose: Mouse anti-Histone3.3 G34V IgG

Specificity: Knockout verfied

Cross-Reactivity (Details): Reacts with: human (P84243). Other species not tested yet.

Purification: purified IgG

Immunogen: Synthetic peptide corresponding to AA 29 to 38 from human H3F3A G34V (UniProt Id: P84243)

Isotype: IgG2b

Application Details

Application Notes: WB:not recommended IP:not tested yet ICC:not tested yet IHC:not tested yet IHC-P:1 : 1000 up to 1 : 2000, ChIP:external data

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Do not freeze!

Target Details for Histone H3.3

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Alternative Name: Histone3.3

Background:

Synonyms: Histon 3-3 G34V, H3F3A, G34V, nucleus

Antigen Information: K.O. Histone 3.3 G34V is a driver mutation in paediatric glioblastoma

UniProt: P84243