Arylsulfatase A antibody (C-Term)

Catalog No. ABIN5692787

This anti-Arylsulfatase A antibody is a Mouse Monoclonal antibody detecting Arylsulfatase A in WB, IHC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for Arylsulfatase A antibody (C-Term) (ABIN5692787)

Target: Arylsulfatase A (ARSA)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Arylsulfatase A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 4C10

Product Details anti-Arylsulfatase A Antibody

Binding Specificity: AA 454-482, C-Term

Purpose: Anti-ARSA Antibody Picoband® (monoclonal, 4C10)

Sequence: QALKQLQLLK AQLDAAVTFG PSQVARGED

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ARSA Antibody Picoband® (monoclonal, 4C10) (ABIN5692787). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ARSA, different from the related mouse sequence by six amino acids.

Isotype: IgG2a

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse
1. Fujii T, Kobayashi T, Honke K et al. (1992). "Proteolytic processing of human lysosomal arylsulfatase A". Biochim. Biophys. Acta 1122 (1): 93-8. 2. Kondo R, Wakamatsu N, Yoshino H et al. (1991)."Identification of a mutation in the arylsulfatase A gene of a patient with adult-type metachromatic leukodystrophy". Am. J. Hum. Genet. 48 (5): 971-8. 3. Matzner U, Herbst E, Hedayati KK, Lüllmann-Rauch R, Wessig C, Schröder S, Eistrup C, Möller C, Fogh J, Gieselmann V (May 2005). "Enzyme replacement improves nervous system pathology and function in a mouse model for metachromatic leukodystrophy". Hum. Mol. Genet. 14 (9): 1139-52.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Arylsulfatase A

Target: Arylsulfatase A (ARSA)

Alternative Name: ARSA

Background:

Synonyms: Arylsulfatase A, ASA, Cerebroside-sulfatase, Arylsulfatase A component B, Arylsulfatase A component C, ARSA

Background: Arylsulfatase A (ARSA) is an enzyme that breaks down sulfatides, namely cerebroside 3-sulfate intocerebroside and sulfate. In humans, arylsulfatase A is encoded by the ARSA gene. ARSA is mapped to 22q13.33. The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene.

Molecular Weight: 54 kDa

Gene ID: 410

UniProt: P15289