ACTN3 antibody (C-Term) (DyLight 488)

Catalog No. ABIN7354740

This Rabbit Polyclonal antibody specifically detects ACTN3 in FACS. It exhibits reactivity toward Human.

Quick Overview for ACTN3 antibody (C-Term) (DyLight 488) (ABIN7354740)

Target: ACTN3 (Actinin, alpha 3 (ACTN3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTN3 antibody is conjugated to DyLight 488

Application: Flow Cytometry (FACS)

Product Details anti-ACTN3 Antibody (DyLight 488)

Binding Specificity: AA 574-617, C-Term

Purpose: Anti-Human ACTN3 DyLight® 488 conjugated Antibody

Sequence: EADRERGAIM GIQGEIQKIC QTYGLRPCST NPYITLSPQD INT

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human ACTN3 DyLight® 488 conjugated Antibody -Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ACTN3, different from the related mouse sequence by five amino acids.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Papadimitriou ID, Papadopoulos C, Kouvatsi A, Triantaphyllidis C (April 2008). "The ACTN3 gene in elite Greek track and field athletes". Int J Sports Med 29 (4): 352-5. 2. Yang N, MacArthur DG, Gulbin JP, Hahn AG, Beggs AH, Easteal S, North K (September 2003). "ACTN3 genotype is associated with human elite athletic performance". Am. J. Hum. Genet. 73 (3): 627-31.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for ACTN3

Target: ACTN3 (Actinin, alpha 3 (ACTN3))

Alternative Name: ACTN3

Background:

Synonyms: Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein, ACTN3

Tissue Specificity: Expressed only in a subset of type 2 skeletal muscle fibers.

Background: Alpha-actinin-3, also known as alpha-actinin skeletal muscle isoform 3 or F-actin cross-linking protein, is a protein that in humans is encoded by the ACTN3 gene. This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants, the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.

Molecular Weight: 39 kDa

Gene ID: 89

UniProt: Q08043

Pathways: Cell-Cell Junction Organization