ACTN3 antibody (N-Term)

Catalog No. ABIN2777061

This Rabbit Polyclonal antibody specifically detects ACTN3 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig, Horse and Zebrafish (Danio rerio).

Quick Overview for ACTN3 antibody (N-Term) (ABIN2777061)

Target: ACTN3 (Actinin, alpha 3 (ACTN3))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTN3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ACTN3 Antibody

Binding Specificity: N-Term

Purpose: ACTN3 Antibody - N-terminal region

Sequence: VQNFHTSWKD GLALCALIHR HRPDLIDYAK LRKDDPIGNL NTAFEVAEKY

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against ACTN3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ACTN3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP41914-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ACTN3

Target: ACTN3 (Actinin, alpha 3 (ACTN3))

Alternative Name: ACTN3

Background:

Background Information: Alpha-actinin is an actin-binding protein with multiple roles in different cell types. This protein expression is limited to skeletal muscle. It is localized to the Z-disc and analogous dense bodies, where it helps to anchor the myofibrillar actin filaments.Alpha-actinin is an actin-binding protein with multiple roles in different cell types. This gene expression is limited to skeletal muscle. It is localized to the Z-disc and analogous dense bodies, where it helps to anchor the myofibrillar actin filaments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Actinin, alpha 3

Alternative Symbols: ACTN3D

Protein Name: Alpha-actinin-3

Molecular Weight: 103kDa

Gene ID: 89

NCBI Accession: NP_001095

UniProt: Q08043

Pathways: Cell-Cell Junction Organization