C7 antibody (AA 233-453)

Catalog No. ABIN5692807

The Rabbit Polyclonal anti-C7 antibody has been validated for WB and ELISA. It is suitable to detect C7 in samples from Human, Mouse and Rat.

Quick Overview for C7 antibody (AA 233-453) (ABIN5692807)

Target: C7 (Complement Component C7 (C7))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-C7 Antibody

Binding Specificity: AA 233-453

Purpose: Anti-Complement C7 Antibody Picoband®

Brand: Picoband™

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Complement C7 detection. Tested with WB, Direct ELISA in Human,Mouse,Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Complement C7 recombinant protein (Position: R233-D453).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Barroso, S., Rieubland, C., Alvarez, A. J., Lopez-Trascasa, M., Bart, P.-A., Nunez-Roldan, A., Sanchez, B. Molecular defects of the C7 gene in two patients with complement C7deficiency. Immunology 118: 257-260, 2006. 2. Coto, E., Martinez-Naves, E., Dominguez, O., DiScipio, R. G., Urra, J. M., Lopez-Larrea, C.DNA polymorphism and linkage relationship of the human complement component C6,C7, and C9 genes. Immunogenetics 33: 184-187, 1991.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for C7

Target: C7 (Complement Component C7 (C7))

Alternative Name: C7

Background:

Synonyms: Complement component C7, C7

Tissue Specificity: Widely expressed.

Background: This gene encodes a serum glycoprotein that forms a membrane attack complex together with complement components C5b, C6, C8, and C9 as part of the terminal complement pathway of the innate immune system. The protein encoded by this gene contains a cholesterol-dependent cytolysin/membrane attack complex/perforin-like (CDC/MACPF) domain and belongs to a large family of structurally related molecules that form pores involved in host immunity and bacterial pathogenesis. This protein initiates membrane attack complex formation by binding the C5b-C6 subcomplex and inserts into the phospholipid bilayer, serving as a membrane anchor. Mutations in this gene are associated with a rare disorder called C7 deficiency.

Molecular Weight: 93 kDa

Gene ID: 730

UniProt: P10643

Pathways: Complement System