Emerin antibody (N-Term)

Catalog No. ABIN5692852

The Mouse Monoclonal anti-Emerin antibody has been validated for WB, IHC, ICC and FACS. It is suitable to detect Emerin in samples from Human.

Quick Overview for Emerin antibody (N-Term) (ABIN5692852)

Target: Emerin (EMD)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Emerin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 5A10

Product Details anti-Emerin Antibody

Binding Specificity: AA 1-48, N-Term

Purpose: Anti-Emerin EMD Antibody (monoclonal, 5A10)

Sequence: MDNYADLSDT ELTTLLRRYN IPHGPVVGST RRLYEKKIFE YETQRRRL

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Emerin EMD Antibody (monoclonal, 5A10) (ABIN5692852). Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Emerin, different from the related mouse sequence by eight amino acids, and from the related rat sequence by nine amino acids.

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Emerin

Target: Emerin (EMD)

Alternative Name: EMD

Background:

Synonyms: Emerin, EMD, EDMD, STA

Tissue Specificity: Skeletal muscle, heart, colon, testis, ovary and pancreas.

Background: APOBEC3G (apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G) is a human enzyme encoded by the APOBEC3G gene. This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by this gene has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity.

Molecular Weight: 34 kDa

Gene ID: 2010

UniProt: P50402