GLO1 antibody (AA 2-184)

Catalog No. ABIN5692862

This anti-GLO1 antibody is a Rabbit Polyclonal antibody detecting GLO1 in WB, IHC, ELISA and IP. Suitable for Human, Mouse and Rat.

Quick Overview for GLO1 antibody (AA 2-184) (ABIN5692862)

Target: GLO1 (Glyoxalase I (GLO1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Product Details anti-GLO1 Antibody

Binding Specificity: AA 2-184

Purpose: Anti-GLO1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GLO1 Antibody Picoband® (ABIN5692862). Tested in ELISA, IHC, IP, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human GLO1 recombinant protein (Position: A2-M184).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunoprecipitation, 0.5-2 μg/mL, Human
ELISA, 0.1-0.5 μg/mL
1. Wu, Y.-Y., Chien, W.-H., Huang, Y.-S., Gau, S. S.-F., Chen, C.-H. Lack of evidence to support the glyoxalase 1 gene (GLO1) as a risk gene of autism in Han Chinese patients from Taiwan. Prog. Neuropsychopharmacol. Biol. Psychiatry 32: 1740-1744, 2008. 2. Ziegler, A., Fonatsch, C., Kompf, J. Mapping of the locus for glyoxalase 1 (GLO1) on human chromosome 6 using mutant cell lines. (Abstract) Cytogenet. Cell Genet. 40: 787 only, 1985.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for GLO1

Target: GLO1 (Glyoxalase I (GLO1))

Alternative Name: GLO1

Background:

Synonyms: Lactoylglutathione lyase, Aldoketomutase, Glyoxalase I, Glx I, Ketone-aldehyde mutase, Methylglyoxalase, S-D-lactoylglutathione methylglyoxal lyase, GLO1

Tissue Specificity: Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes.

Background: Lactoylglutathione lyase in humans is encoded by the GLO1 gene. The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.

Molecular Weight: 21 kDa

Gene ID: 2739

UniProt: Q04760