Glycerol Kinase antibody (AA 1-180)

Catalog No. ABIN5692865

This anti-Glycerol Kinase antibody is a Rabbit Polyclonal antibody detecting Glycerol Kinase in ELISA, WB and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for Glycerol Kinase antibody (AA 1-180) (ABIN5692865)

Target: Glycerol Kinase (GK)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glycerol Kinase antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Glycerol Kinase Antibody

Binding Specificity: AA 1-180

Purpose: Anti-Glycerol kinase/GK Antibody Picoband®

Brand: Picoband™

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Glycerol kinase detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Immunogen: E. coli-derived human Glycerol kinase recombinant protein (Position: M1-S180).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Dipple, K. M., Zhang, Y.-H., Huang, B.-L., McCabe, L. L., Dallongeville, J., Inokuchi, T., Kimura, M., Marx, H. J., Roederer, G. O., Shih, V., Yamaguchi, S., Yoshida, I., McCabe, E. R. B.Glycerol kinase deficiency: evidence for complexity in a single gene disorder. Hum. Genet. 109: 55-62, 2001. 2. Gaudet, D., Arsenault, S., Perusse, L., Vohl, M.-C., St.-Pierre, J., Bergeron, J., Despres, J.-P., Dewar, K., Daly, M. J., Hudson, T., Rioux, J. D. Glycerol as a correlate of impaired glucose tolerance: dissection of a complex system by use of a simple genetic trait. Am. J. Hum. Genet. 66: 1558-1568, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Glycerol Kinase

Target: Glycerol Kinase (GK)

Alternative Name: GK

Background:

Synonyms: Glycerol kinase, GK, Glycerokinase, ATP:glycerol 3-phosphotransferase, GK

Tissue Specificity: Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.

Background: Glycerol kinase is a phosphotransferase enzyme involved in triglycerides and glycerophospholipids synthesis. The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 61 kDa

Gene ID: 2710

UniProt: P32189