RAB27A antibody (AA 98-216)

Catalog No. ABIN5692927

The Rabbit Polyclonal anti-RAB27A antibody has been validated for WB, ELISA, IHC, FACS and ICC. It is suitable to detect RAB27A in samples from Human, Mouse and Rat.

Quick Overview for RAB27A antibody (AA 98-216) (ABIN5692927)

Target: RAB27A (RAB27A, Member RAS Oncogene Family (RAB27A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB27A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-RAB27A Antibody

Binding Specificity: AA 98-216

Purpose: Anti-RAB27A Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RAB27A Antibody (ABIN5692927). Tested in ELISA, Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human RAB27A recombinant protein (Position: L98-K216).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RAB27A

Target: RAB27A (RAB27A, Member RAS Oncogene Family (RAB27A))

Alternative Name: RAB27A

Background:

Synonyms: Ras-related protein Rab-27A, Rab-27, GTP-binding protein Ram, RAB27A, RAB27

Tissue Specificity: Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.

Background: Ras-related protein Rab-27A is a protein that in humans is encoded by the RAB27A gene. The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.

Molecular Weight: 27 kDa

Gene ID: 5873

UniProt: P51159

Pathways: Peptide Hormone Metabolism