ICAM1 antibody (AA 28-268)

Catalog No. ABIN5692991

This anti-ICAM1 antibody is a Rabbit Polyclonal antibody detecting ICAM1 in WB, FACS, IHC, ELISA, IF and ICC. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 18+ publications.

Quick Overview for ICAM1 antibody (AA 28-268) (ABIN5692991)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ICAM1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ICAM1 Antibody

Binding Specificity: AA 28-268

Purpose: Anti-ICAM1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ICAM1 Antibody Picoband® (ABIN5692991). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human ICAM1 recombinant protein (Position: Q28-R268).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Ballantyne, C. M., Kozak, C. A., O'Brien, W. E., Beaudet, A. L. : Assignment of the gene for intercellular adhesion molecule-1 (Icam-1) to proximal mouse chromosome 9. Genomics 9: 547-550, 1991. 2. Greve, J. M., Davis, G., Meyer, A. M., Forte, C. P., Yost, S. C., Marlor, C. W., Kamarck, M. E., McClelland, A. : The major human rhinovirus receptor is ICAM-1. Cell 56: 839-847, 1989. 3. Prieto, J., Takei, F., Gendelman, R., Christenson, B., Biberfeld, P., Patarroyo, M. : MALA-2, mouse homologue of human adhesion molecule ICAM-1 (CD54). Europ. J. Immun. 19: 1551-1557, 1989.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-ICAM1 antibody (ABIN5692991)

Ma, Pan, Ren, Guo, Guo, Wei, Zheng, Chen: "15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell." in: Lipids in health and disease, Vol. 16, Issue 1, pp. 137, (2018) (PubMed).

Ma, Pan, Chen, Guo, Zhao, Zheng, Chen: "Trimethylamine N-oxide in atherogenesis: impairing endothelial self-repair capacity and enhancing monocyte adhesion." in: Bioscience reports, Vol. 37, Issue 2, (2017) (PubMed).

Wang, Sun, Shen, Wu, Liu, Yang, Ji, Ji, Li: "Octreotide Protects the Mouse Retina against Ischemic Reperfusion Injury through Regulation of Antioxidation and Activation of NF-κB." in: Oxidative medicine and cellular longevity, Vol. 2015, pp. 970156, (2016) (PubMed).

Pan, Wang, Pu, Yao, Wang: "Effect of Puerarin on Expression of ICAM-1 and TNF-α in Kidneys of Diabetic Rats." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 2134-40, (2016) (PubMed).

Wang, Wang, Li, Wu, Liu, Yang, Ji, Ji, Li: "The Neuropilin-1 Inhibitor, ATWLPPR Peptide, Prevents Experimental Diabetes-Induced Retinal Injury by Preserving Vascular Integrity and Decreasing Oxidative Stress." in: PLoS ONE, Vol. 10, Issue 11, pp. e0142571, (2016) (PubMed).

Cheng, Zhang, Song, Feng: "Intercellular adhesion molecule-1 expression in the hippocampal CA1 region of hyperlipidemic rats with chronic cerebral ischemia." in: Neural regeneration research, Vol. 7, Issue 17, pp. 1312-7, (2015) (PubMed).

Chen, Zhang, Chen, Liu, Wang, Zhou, Qiu, Zhang, Teng, Mao, Liu, Wei, Li: "Retinoic Acid promotes interleukin-4 plasmid-dimethylsulfoxide topical transdermal delivery for treatment of psoriasis." in: Annals of dermatology, Vol. 27, Issue 2, pp. 121-7, (2015) (PubMed).

Ning, Liu, Lv, Yang, Zhang, Yu, Wang, Zhu: "Lipid metabolism and inflammation modulated by Vitamin D in liver of diabetic rats." in: Lipids in health and disease, Vol. 14, pp. 31, (2015) (PubMed).

Fan, Yao, Li, Hu, Shao, Tian: "Anti-inflammatory and antioxidant effects of curcumin on acute lung injury in a rodent model of intestinal ischemia reperfusion by inhibiting the pathway of NF-Kb." in: International journal of clinical and experimental pathology, Vol. 8, Issue 4, pp. 3451-9, (2015) (PubMed).

Wang, Zhao, Fang, Li, Shen, Cao, Zhu: "Glycyrrhizin protects against porcine endotoxemia through modulation of systemic inflammatory response." in: Critical care, Vol. 17, Issue 2, pp. R44, (2014) (PubMed).

Fan, Jing, Yao, Li, Hu, Shao, Shen, Pan, Luo, Tian: "The protective effects of curcumin on experimental acute liver lesion induced by intestinal ischemia-reperfusion through inhibiting the pathway of NF-?B in a rat model." in: Oxidative medicine and cellular longevity, Vol. 2014, pp. 191624, (2014) (PubMed).

Yu, Chen, Wang, Kuang, Liu, Zhang, Du: "Neuroprotective effect of kaempferol glycosides against brain injury and neuroinflammation by inhibiting the activation of NF-?B and STAT3 in transient focal stroke." in: PLoS ONE, Vol. 8, Issue 2, pp. e55839, (2013) (PubMed).

Yu, Chen, Jiang: "Administration of pigment epithelium-derived factor delivered by adeno-associated virus inhibits blood-retinal barrier breakdown in diabetic rats." in: Molecular vision, Vol. 16, pp. 2384-94, (2011) (PubMed).

Yao, Zhang, Zheng, Li, Wang, Wang, Chu, Hu, Liu, Tian: "Prophylaxis with carnosol attenuates liver injury induced by intestinal ischemia/reperfusion." in: World journal of gastroenterology, Vol. 15, Issue 26, pp. 3240-5, (2009) (PubMed).

Wang, Qiao, Li, Yang: "Ginsenoside Rb1 attenuates intestinal ischemia-reperfusion- induced liver injury by inhibiting NF-kappaB activation." in: Experimental & molecular medicine, Vol. 40, Issue 6, pp. 686-98, (2008) (PubMed).

Tian, Yao, Li, Zhang, Feng, Yang, Zheng: "Effect of nuclear factor kappa B on intercellular adhesion molecule-1 expression and neutrophil infiltration in lung injury induced by intestinal ischemia/reperfusion in rats." in: World journal of gastroenterology, Vol. 12, Issue 3, pp. 388-92, (2006) (PubMed).

Hang, Shi, Li, Li, Yin: "Up-regulation of intestinal nuclear factor kappa B and intercellular adhesion molecule-1 following traumatic brain injury in rats." in: World journal of gastroenterology, Vol. 11, Issue 8, pp. 1149-54, (2005) (PubMed).

Zhao, Chen, Zhou, Zhou, Li: "Influence of platelet activating factor on expression of adhesion molecules in experimental pancreatitis." in: World journal of gastroenterology, Vol. 9, Issue 2, pp. 338-41, (2003) (PubMed).

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: ICAM1

Background:

Synonyms: Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, CD54, ICAM1

Tissue Specificity: Present in T-cells, monocytes, epithelial cells and hepatocytes (at protein level). Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes. .

Background: CD54, also known as ICAM-1. Intercellular adhesion molecule-1 (ICAM1) is a ligand for lymphocyte function-associated (LFA) antigens. ICAM-1 is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for LFA-1, a beta 2 leukocyte integrin. This protein is the major human rhinovirus receptor. The ICAM1 gene is mapped to human chromosome 19. In humans, lymphocyte adhesion to cells is mediated by the protein heterodimer CD11a/CD18 (Leu-CAMa, LFA-1) and its ligand CD54 (ICAM-1).

Molecular Weight: 90 kDa

Gene ID: 3383

UniProt: P05362

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling