CCKBR antibody (DyLight 488)

Catalog No. ABIN7354665

This anti-CCKBR antibody is a Rabbit Polyclonal antibody detecting CCKBR in FACS. Suitable for Human.

Quick Overview for CCKBR antibody (DyLight 488) (ABIN7354665)

Target: CCKBR (Cholecystokinin B Receptor (CCKBR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCKBR antibody is conjugated to DyLight 488

Application: Flow Cytometry (FACS)

Product Details anti-CCKBR Antibody (DyLight 488)

Purpose: Anti-Human CCKBR DyLight® 488 conjugated Antibody

Sequence: PVYTVVQPVG PRVLQCVHRW PSARVRQTWS

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human CCKBR DyLight® 488 conjugated Antibody -Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human CCKBR, which shares 80% and 90% amino acid (aa) sequence identity with mouse and rat CCKBR, respectively.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Altar CA, Boyar WC (Apr 1989). "Brain CCK-B receptors mediate the suppression of dopamine release by cholecystokinin". Brain Research. 483 (2): 321-6. 2. Noble F, Roques BP (Jul 1999). "CCK-B receptor: chemistry, molecular biology, biochemistry and pharmacology". Progress in Neurobiology. 58 (4): 349-79.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for CCKBR

Target: CCKBR (Cholecystokinin B Receptor (CCKBR))

Alternative Name: CCKBR

Background:

Synonyms: Gastrin/cholecystokinin type B receptor, CCK-B receptor, CCK-BR, Cholecystokinin-2 receptor, CCK2-R, CCKBR, CCKRB

Tissue Specificity: Isoform 1 is expressed in brain, pancreas, stomach, the colon cancer cell line LoVo and the T-lymphoblastoma Jurkat, but not in heart, placenta, liver, lung, skeletal muscle, kidney or the stomach cancer cell line AGS. Expressed at high levels in the small cell lung cancer cell line NCI-H510, at lower levels in NCI-H345, NCI-H69 and GLC-28 cell lines, not expressed in GLC-19 cell line. Within the stomach, expressed at high levels in the mucosa of the gastric fundus and at low levels in the antrum and duodenum. Isoform 2 is present in pancreatic cancer cells and colorectal cancer cells, but not in normal pancreas or colonic mucosa. Isoform 3 is expressed in brain, pancreas, stomach, the stomach cancer cell line AGS and the colon cancer cell line LoVo.

Background: The cholecystokinin B receptor, also known as CCKBR or CCK2, is a protein that in humans is encoded by the CCKBR gene. This gene encodes a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. Alternative splicing results in multiple transcript variants. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors.

Molecular Weight: 39 kDa

Gene ID: 887

UniProt: P32239

Pathways: Positive Regulation of Peptide Hormone Secretion, Feeding Behaviour