HTRA1 antibody

Catalog No. ABIN5692999

The Rabbit Polyclonal anti-HTRA1 antibody is suitable to detect HTRA1 in samples from Human, Mouse and Rat. It has been validated for WB and IHC.

Quick Overview for HTRA1 antibody (ABIN5692999)

Target: HTRA1 (HtrA Serine Peptidase 1 (HTRA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HTRA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HTRA1 Antibody

Purpose: Anti-HTRA1 Antibody

Sequence: QLRAASRRSE RLHRPPVIVL QRGACGQGQE DPNSLRHKYN FIAD

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HTRA1 Antibody (ABIN5692999). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HTRA1, which shares 93.2% amino acid (aa) sequence identity with both mouse and rat HTRA1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HTRA1

Target: HTRA1 (HtrA Serine Peptidase 1 (HTRA1))

Alternative Name: HTRA1

Background:

Synonyms: Serine protease HTRA1, High-temperature requirement A serine peptidase 1, L56, Serine protease 11, HTRA1, HTRA, PRSS11

Tissue Specificity: Widely expressed, with strongest expression in placenta (at protein level). Secreted by synovial fibroblasts. Up- regulated in osteoarthritis and rheumatoid arthritis synovial fluids and cartilage as compared with non-arthritic (at protein level).

Background: Serine protease HTRA1 is an enzyme that in humans is encoded by the HTRA1 gene. This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7.

Molecular Weight: 51 kDa

Gene ID: 5654

UniProt: Q92743

Pathways: Growth Factor Binding