POR antibody (C-Term) (DyLight 488)

Catalog No. ABIN7354705

This anti-POR antibody is a Rabbit Polyclonal antibody detecting POR in FACS. Suitable for Human.

Quick Overview for POR antibody (C-Term) (DyLight 488) (ABIN7354705)

Target: POR (P450 (Cytochrome) Oxidoreductase (POR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POR antibody is conjugated to DyLight 488

Application: Flow Cytometry (FACS)

Product Details anti-POR Antibody (DyLight 488)

Binding Specificity: AA 633-668, C-Term

Purpose: Anti-Human POR DyLight® 488 conjugated Antibody

Sequence: RNMARDVQNT FYDIVAELGA MEHAQAVDYI KKLMTK

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human POR DyLight® 488 conjugated Antibody -Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human POR , different from the related mouse and rat sequences by five amino acids.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Adachi, M., Asakura, Y., Matsuo, M., Yamamoto, T., Hanaki, K., Arlt, W. POR R457H is a global founder mutation causing Antley-Bixler syndrome with autosomal recessive trait. (Letter) Am. J. Med. Genet. 140A: 633-635, 2006. 2. Miller, W. L. Congenital adrenal hyperplasia. (Letter) New Eng. J. Med. 314: 1321-1322, 1986. 3. Shephard, E. A., Phillips, I. R., Santisteban, I., West, L. F., Palmer, C. N., Ashworth, A., Povey, S. Isolation of a human cytochrome P-450 reductase cDNA clone and localization of the corresponding gene to chromosome 7q11.2. Ann. Hum. Genet. 53: 291-301, 1989.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for POR

Target: POR (P450 (Cytochrome) Oxidoreductase (POR))

Alternative Name: POR

Background:

Synonyms: NADPH--cytochrome P450 reductase

Tissue Specificity: Highly expressed in adult liver, lung and spleen than in corresponding fetal tissue. Also expressed in the lymph node, placenta, spinal cord and heart tissues. Expression is more elevated in peripheral blood leukocytes than in the bone marrow and in normal cells than malignant cells. Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Strongly expressed in acute inflammatory lesions caused by bacteria and fungi. Isoform 2 was detected in the lung, liver and mature monocytes.

Background: POR is a membrane-boundenzyme required for electron transfer from NADPH to cytochrome P450 in the endoplasmic reticulum of theeukaryotic cell. The gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons ly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.

Molecular Weight: 39 kDa

Gene ID: 5447

UniProt: P16435

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, SARS-CoV-2 Protein Interactome