GAPDH antibody

Catalog No. ABIN5693113

The Rabbit Monoclonal anti-GAPDH antibody has been validated for WB. It is suitable to detect GAPDH in samples from Human, Mouse, Rat and Monkey. There are 46+ publications available.

Quick Overview for GAPDH antibody (ABIN5693113)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB)

Clone: A-7

Product Details anti-GAPDH Antibody

Purpose: Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated

Characteristics: Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated (ABIN5693113). Tested in WB application. This antibody reacts with Human, Monkey, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human GAPDH (HRP conjugated)

Isotype: IgG

Application Details

Application Notes: WB 1:5000-1:20000

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

References for anti-GAPDH antibody (ABIN5693113)

Wang, Xue, Zhang, Bu, Zhu, Lai: "Autophagy protects ovarian cancer-associated fibroblasts against oxidative stress." in: Cell cycle (Georgetown, Tex.), Vol. 15, Issue 10, pp. 1376-85, (2018) (PubMed).

Yang, Zhang, Li, Wen: "MALAT1 enhanced the proliferation of human osteoblasts treated with ultra‑high molecular weight polyethylene by targeting VEGF via miR‑22‑5p." in: International journal of molecular medicine, Vol. 41, Issue 3, pp. 1536-1546, (2018) (PubMed).

Liu, Mei, Xu, Yu, Shi, Zhang, Wang, Zhang, Gao, Zhang, He: "Dual Receptor Recognizing Cell Penetrating Peptide for Selective Targeting, Efficient Intratumoral Diffusion and Synthesized Anti-Glioma Therapy." in: Theranostics, Vol. 6, Issue 2, pp. 177-91, (2017) (PubMed).

Zhu, Ren, Zhou, Cheng: "The combination of blueberry juice and probiotics reduces apoptosis of alcoholic fatty liver of mice by affecting SIRT1 pathway." in: Drug design, development and therapy, Vol. 10, pp. 1649-61, (2017) (PubMed).

Feng, Li, Chen, Liu, Rong, Wang, Du: "Combined effects of interleukin-1β and cyclic stretching on metalloproteinase expression in corneal fibroblasts in vitro." in: Biomedical engineering online, Vol. 15, Issue 1, pp. 63, (2017) (PubMed).

Dong, Liu, Zhang, Xu, Qin, Cheng, Huang, Guo, Yang, Chen: "Inhibition of SDF-1α/CXCR4 Signalling in Subchondral Bone Attenuates Post-Traumatic Osteoarthritis." in: International journal of molecular sciences, Vol. 17, Issue 6, (2017) (PubMed).

Jing, Heng, Xia, Ning, Yafei, Yao, Shulan: "Downregulation of phosphoglycerate dehydrogenase inhibits proliferation and enhances cisplatin sensitivity in cervical adenocarcinoma cells by regulating Bcl-2 and caspase-3." in: Cancer biology & therapy, Vol. 16, Issue 4, pp. 541-8, (2016) (PubMed).

Qian, Li, Yu, Yang, Deng, Ji, Jiao, Li, Wu, Chen, Feng, Zhu: "Inhibition of DNA methyltransferase as a novel therapeutic strategy to overcome acquired resistance to dual PI3K/mTOR inhibitors." in: Oncotarget, Vol. 6, Issue 7, pp. 5134-46, (2016) (PubMed).

Wang, Ge, Chen, Hu, Li, Song, Xu, Ye: "A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21 (Waf1/CIP1) Expression." in: BioMed research international, Vol. 2015, pp. 304753, (2016) (PubMed).

Li, Qian, Yu, Li, Wu, Ji, Jiao, Li, Kong, Chen, Feng, Deng, Zhu: "EGFR tyrosine kinase inhibitors promote pro-caspase-8 dimerization that sensitizes cancer cells to DNA-damaging therapy." in: Oncotarget, Vol. 6, Issue 19, pp. 17491-500, (2016) (PubMed).

Tang, Wang, Chen, Xu, Ye: "Clinicopathologic and prognostic significance of p21 (Cip1/Waf1) expression in bladder cancer." in: International journal of clinical and experimental pathology, Vol. 8, Issue 5, pp. 4999-5007, (2016) (PubMed).

Zhu, Liu, Wang, Nie, He, Zhang, Liu, Su: "Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats." in: Molecular medicine reports, Vol. 12, Issue 4, pp. 5691-700, (2016) (PubMed).

Guo, Tian, Dong, Bai, Yang: "Knockdown of Gli1 by small-interfering RNA enhances the effects of BCNU on the proliferation and apoptosis of glioma U251 cells." in: International journal of clinical and experimental pathology, Vol. 8, Issue 7, pp. 7762-73, (2016) (PubMed).

Li, He, Han, Hu: "Dasatinib suppresses invasion and induces apoptosis in nasopharyngeal carcinoma." in: International journal of clinical and experimental pathology, Vol. 8, Issue 7, pp. 7818-24, (2016) (PubMed).

Lin, Chen, Zhang, Lin, Liu, Li, Zeng, Chen, Du, Chen, Tan, Liu: "IL-10 Protects Neurites in Oxygen-Glucose-Deprived Cortical Neurons through the PI3K/Akt Pathway." in: PLoS ONE, Vol. 10, Issue 9, pp. e0136959, (2016) (PubMed).

Ding, Lei, Han, Li, Qu, Fu, Fu, Wang: "SIRT1 protects against myocardial ischemia-reperfusion injury via activating eNOS in diabetic rats." in: Cardiovascular diabetology, Vol. 14, pp. 143, (2016) (PubMed).

Chen, Liu, Tian, Wu, Ai, Tang, Zhao, Ding, Zhang, Tang: "miR-124 and miR-506 inhibit colorectal cancer progression by targeting DNMT3B and DNMT1." in: Oncotarget, Vol. 6, Issue 35, pp. 38139-50, (2016) (PubMed).

Han, Shen, Chang: "Involvement of epithelial-to-mesenchymal transition and associated transforming growth factor-β/Smad signaling in paraquat-induced pulmonary fibrosis." in: Molecular medicine reports, Vol. 12, Issue 6, pp. 7979-84, (2016) (PubMed).

Liao, Xue, Zhang, Feng, Liu, Zhang: "Higher heat shock factor 1 expression in tumor stroma predicts poor prognosis in esophageal squamous cell carcinoma patients." in: Journal of translational medicine, Vol. 13, pp. 338, (2016) (PubMed).

Liu, Gao, Yao, Gong: "Quercetin Alleviates High-Fat Diet-Induced Oxidized Low-Density Lipoprotein Accumulation in the Liver: Implication for Autophagy Regulation." in: BioMed research international, Vol. 2015, pp. 607531, (2016) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background:

Synonyms: Glyceraldehyde-3-phosphate dehydrogenase,GAPDH,1.2.1.12,Peptidyl-cysteine S-nitrosylase GAPDH,2.6.99.-,GAPDH,GAPD,CDABP0047, OK/SW-cl.12,

Tissue Specificity: Detected in brain and placenta.

Molecular Weight: 68 kDa

UniProt: P04406